Introduction
Post-translation modification are a way for a protein to modify its functionality through methods such as the addition or breaking of covalent bonds [1]. This includes things like phosphorylation which is the addition of a phosphate to a protein. Phosphorylation is one way that proteins can modify activity in relation to cell signaling. Other types of modifications include glycosylation, acetylation, methylation, and ubiquitination.
results
Predicted phosphorylation sites for the COL1A1 protein in Zebrafish
Conclusion
The image above shows the predicted phosphorylation sites for the COL1A1 protein in Zebrafish which is the focus of my specific aims to study vEDS. This prediction was created by using the NetPhos - 3.1 program [2]. Predicted kinases include ATM, PKA, PKC, and cdk5. While none of my proposed experiments correlate directly with phosphorylation sites, this could be an interesting avenue to explore.
References
[1] Zhong Q, Xiao X, Qiu Y, et al. Protein posttranslational modifications in health and diseases: Functions, regulatory mechanisms, and therapeutic implications. MedComm (2020). 2023;4(3):e261. Published 2023 May 2. doi:10.1002/mco2.261
[2] https://services.healthtech.dtu.dk/services/NetPhos-3.1/
[2] https://services.healthtech.dtu.dk/services/NetPhos-3.1/
This web page was produced as an assignment for Genetics 564, a capstone course at UW-Madison